|
KMID : 1007520140230051643
|
|
Food Science and Biotechnology
2014 Volume.23 No. 5 p.1643 ~ p.1651
|
|
|
The Effects of Fucosylated Chondroitin Sulfate Isolated from Isostichopus badionotus on Antimetastatic Activity via Downregulation of Hif-1α and Hpa
|
|
He Min
Wang Jingfeng
Hu Shiwei
Wang Yuming
Xue Changhu
Li Hui
|
|
|
Abstract
|
|
|
|
Fucosylated chondroitin sulfate (CHS) was prepared from the sea cucumber Isostichopus badionotus (Ib-CHS), and the antimetastatic effect was studied using cell adhesion, migration, invasion, chorioallntoic membrane (CAM), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays. Ib-CHS remarkably suppressed proliferation of 95D cells, and inhibited adhesion, migration, and invasion of 95D cells in a dose-dependent manner. CAM assay results suggested that Ib-CHS attenuates neovascularization in the chick embryo. RT-PCR analyses revealed that Ib- CHS dramatically decreased expression of matrix metalloproteinase- 2/9 (MMP-2/9), hypoxia-inducible factor (Hif- 1α), heparanase (Hpa), and vascular endothelial growth factor (VEGF), and increased expression levels of the tissue inhibitors of metalloproteinase-1/2 (TIMP-1/2), important inhibitors of MMP-2/9. Ib-CHS markedly inhibited tumor growth, improved the metastatic foci occurrence rate, and down-regulated expressions of Hif-1α, Hpa, and VEGF in vivo. Ib-CHS exerted antimetastatic activities in vitro and in vivo.
|
|
|
KEYWORD
|
|
|
fucosylated chondroitin sulfate, antimetastasis, hypoxia-inducible factor-1, heparanase, vascular endothelial growth factor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|